antimyeloperoxidase

To develop an animal model for antimyeloperoxidase (MPO)-associated necrotizing crescentic glomerulonephritis (NCGN), we immunized Brown Norway rats with MPO and localized a neutrophil lysosomal enzyme extract, primarily consisting of MPO and elastinolytic enzymes, plus H₂O₂, the substrate of MPO, to the glomerular basement membrane (GBM).

1993 April, Elisabeth Brouwer, “Antimyeloperoxidase-associated proliferative glomerulonephritis: an animal model”, in Journal of Experimental Medicine, volume 177, number 4, page 905

They were then incubated with the first antibodies: rabbit antimyeloperoxidase antibodies (1:200, RB-373; Thermo Fisher Scientific, Waltham, Massachusetts) for neutrophil staining, rat antimouse Mac-3 antibodies (1:500; BioLegend, San Diego, California) for macrophage staining, rabbit anti-CD3 polyclonal antibodies (1:100; ab5690, Abcam, Cambridgeshire, UK) and rat anti-CD19 monoclonal antibodies (1:500; ab25232, Abcam, Cambridgeshire, UK) for lymphocyte staining.

2015 November 19, “Mechanical Stimulation by Postnasal Drip Evokes Cough”, in PLOS ONE, →DOI