microshoots

Data were recorded after 45 days. –: zero transformation frequency Different types of explants i.e. leaves (approximately 5 × 5 mm size) and nodal segments (approximately 1.5–2 cm in length) from in vitro grown microshoots and in vitro grown callus (approximately 5 × 5 mm size) were taken, cut into small pieces, dipped in 48 h old culture of A. rhizogenes (MTCC 532) and pricked before placing in incubator shaker at 25 °C under shaking condition for different time periods (30 min to 4 h; Table 2 ).

2015 August 22, Latika Brijwal, Sushma Tamta, “Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.”, in SpringerPlus, volume 4, →DOI