misprime

present singular third person misprimes · participle present mispriming · participle past misprimed · past misprimed

1. To prime incorrectly.

   It is also useful when using degenerate primers which may misprime and cause nonspecific products.

   2007, Ralph Rapley, John M. Walker, Molecular Biomethods Handbook, page 310

   2 more examples

   As described previously, primers are apt to dimerize or misprime, particularly in the absence of specific target.

   2010, Stephen A. Bustin, The PCR Revolution: Basic Technologies and Applications, page 139

   Aberrant annealing has also been observed — either due to the use of the incorrect tRNA to misprime reverse transcription (Colicelli and Goff 1986a, b; Wakefield et al. 1995) or due to the priming of cellular mRNAs with short “tRNA-like” primers (Taylor and Cywinski 1984).

   2013, Stuart LeGrice, Matthias Gotte, Human Immunodeficiency Virus Reverse Transcriptase, page 208