semianaerobic

To remove the broad signal arising from the magnetic coupling of the semiquinone to the high spin Fe 2+, the iron was biochemically replaced with diamagnetic Zn 2+ according to the procedures outlined by Utschig et al. 19 Argon gas was slowly blown over the samples to achieve semianaerobic conditions, after which 10 mM dithionite was added to generate SQ A .

2014 July 15, Patrick J. O’Malley, Colin A. Wraight, Sergei A. Dikanov, “Hyperfine and Nuclear Quadrupole Tensors of Nitrogen Donors in the QA Site of Bacterial Reaction Centers: Correlation of the Histidine Nδ Tensors with Hydrogen Bond Strength”, in The Journal of Physical Chemistry. B, volume 118, →DOI