benzonase

plural benzonases

1. countableuncountablebiologyAn artificial endonuclease used to degrade DNA and RNA.

   Treat the lung with benzonase to remove the remnant DNA:

   2011, Charles Y. Lee, Korkut Uygun, Methods in Bioengineering: Organ Preservation and Reengineering, page 185

   5 more examples

   Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob.

   1991 October, J. M. Moreno, “Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase”, in Applied Biochemistry and Biotechnology volume, volume 31

   The volume of benzonase solution (ready-made solution of the manufacturer) necessary to digest the DNA in the pellet III suspension was determined experimentally.

   1999, Andrew J. Link, 2-d Proteome Analysis Protocols, page 83

   Immobilized nucleases like benzonases on corncobs were also used to reduce the nucleic acid content in protein concentrates.

   2000 October, P. Ravindra, Anupama, Value-added food:: Single cell protein, in Biotechnology Advances, volume 18, issue 6, pages 459-479

   Also, in many cases, enzymes like benzonases fail to completely remove nucleic acids from complexes due to inaccessibility of shorter nucleic acids buried in the refolded protein as demonstrated in this study.

   2014 May 23, Muniasamy Neerathilingam, “Soni-removal of nucleic acids from inclusion bodies”, in Biochemical and Biophysical Research Communications, volume 448, number 1, page 45

   The new digestion protocol was designed based on the enzymatic combination of benzonase and P1 nucleases.

   2015, Antonio Monari, Elise Dumont, Chryssostomos Chatgilialoglu, Radiation-induced and oxidative DNA damages, page 42