cytohelicase

The root tips were digested in a pectolytic enzyme mixture [0.1% (w/v) pectolyase Y23 (Duchefa, Haarlem, the Netherlands), 0.1% (w/v) cellulase onozuka RS (Duchefa, Haarlem, the Netherlands) and 0.1% (w/v) cytohelicase (Sigma-Aldrich, Steinheim, Germany)] in 10 mM citrate buffer (10mM tri sodium citrate + 10 mM citrate, pH 4.5) at 37 °C for 1 h.

2015, Prabhu Shankar Lakshmanan et al., “Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae)”, in Comparative Cytogenetics, volume 9, →DOI